ISO 11268-1:1993

Soil quality — Effects of pollutants on earthworms (Eisenia fetida) — Part 1: Determination of acute toxicity using artificial soil substrate

The method described is based on placing adult earthworms in a defined substrate containing the test substance in different concentrations and determining the percent mortality after 7 d and 14 d. It is not applicable to volatile substances, i.e. substances for which Henry’s constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,0133 Pa at 25 °C. It does not take into account the possible degradation of the test substance.