ISO 20948:2024

Vegetable fats and oils — Determination of aflatoxins B1, B2, G1 and G2 by immunoaffinity column clean-up and high-performance liquid chromatography

This document specifies a method for the determination of the aflatoxins B₁, B₂, G₁ and G₂ in vegetable fats and oils, including peanut oil, sesame oil, olive oil, corn oil, sunflower oil, rapeseed oil and coconut oil, using immunoaffinity column clean-up and high-performance liquid chromatography with post-column derivatization.

The limits of quantification for the aflatoxins B₁, B₂, G₁ and G₂, and for the sum of aflatoxins B₁, B₂, G₁ and G₂, are 1?μg/kg, 0,25?μg/kg, 0,5?μg/kg, 0,25?μg/kg and 1?μg/kg, respectively.

The validation was carried out over the following concentration ranges:

—???? aflatoxin B₁?=?1?μg/kg to 20?μg/kg;

—???? total aflatoxins?=?2?μg/kg to 52?μg/kg.

Edition	2024
Published Date	2024
Published By	International Standardization Organisation
Page Count	19