ISO/TS 18220:2016

Water quality — Larval development test with the harpacticoid copepod Nitocra spinipes

ISO/TS 18220:2016 specifies an early-life stage procedure for determination of the toxic effects of chemicals and water samples on a cold-water brackish water copepod species under semi-static conditions. The biological test variables include survival and development of the early-life stages. The exposure starts with newly hatched (<24 h) nauplii (larvae) and is continued until emergence of (c. 50 %) copepodites (juveniles) in the control.

The benthic living Nitocra complements the planktonic Acartia species in ISO 16778. These organisms represent different life-history strategies as Nitocra is egg-carrying, whereas Acartia is a broadcasting calanoid copepod and thus, different sensitivities of specific life stages. Nitocra is a fresh to brackish water species, which allows testing low salinity waters and is complementary to A. tonsa, which is of marine origin and poorly tolerates low salinities.

Edition	2016
Published Date	2016
Published By	ISO/TS
Page Count	20